15mer peptides  (Mimotopes)

Journal: Advanced Science

Article Title: Hydrogen‐Deuterium Exchange Mass Spectrometry for Molecular Glue Characterization

doi: 10.1002/advs.202508543

Figure Lengend Snippet: Protection from hydrogen‐deuterium exchange induced by molecular glue binding. a,b) Uptake plots (i‐vi) of six peptide regions of 14‐3‐3σ displaying % deuterium incorporation in the unbound (black), ERα/LRRK2 bound (pink/purple) and FC‐A stabilized (green) condition. HDX profiles involved with binding partners ERα and LRRK2 are displayed on the left and right, respectively. * represents significant differences between protein complex conditions at 24 h ( p ≤ 0.05). c,d) HDX difference profile of 14‐3‐3/ERα (left) or 14‐3‐3/LRRK2 (right) with and without molecular glue, FC‐A at 24h mapped onto the 14‐3‐3σ structure (PDB: 4JDD (left), 5MY9 (right)). The location of the uptake plots of peptides i‐vi are labelled on the 14‐3‐3 structure.

Article Snippet: Lypholized ERα 581‐595 15mer peptide (Ac‐KYYITGEAEGFPA(pT)V‐COOH, Synpeptide Ltd) and lypholized LRRK2 928‐942 15mer peptide (Ac‐NLQRHSN(pS)LGPIFDH‐CONH 2 , Synpeptide Ltd) were diluted into 100 mM ammonium acetate to make peptide stocks of 1 mM and stored at ‐20 °C prior to use.

Techniques: Binding Assay

Journal: PLOS ONE

Article Title: Human responses to the DNA prime/chimpanzee adenovirus (ChAd63) boost vaccine identify CSP, AMA1 and TRAP MHC Class I-restricted epitopes

doi: 10.1371/journal.pone.0318098

Figure Lengend Snippet: Cohort CA: FluoroSpot IFN-γ and GzB responses of protected participant v90 (HLA A*33:03 (A03)/A*33:0 (A03), B*44:03 (B44)/B*58:01 (B58) to AMA1 Ap8, Ap10 and Ap11 peptide subpools, 15mer peptides, and tested predicted minimal epitopes.

Article Snippet: 15mer peptides were synthesized by Mimotopes, VIC, Australia.

Techniques: Sequencing

Journal: PLOS ONE

Article Title: Human responses to the DNA prime/chimpanzee adenovirus (ChAd63) boost vaccine identify CSP, AMA1 and TRAP MHC Class I-restricted epitopes

doi: 10.1371/journal.pone.0318098

Figure Lengend Snippet: Cohort CA: FluoroSpot IFN-γ and GzB responses for non-protected participant v32 (HLA A*03:03 (A03)/A*03:03) (A03), B*07:02 (B07)/B*07:02 (B07) to AMA1 Ap8 and Ap10 peptide subpools, 15mer peptides, and predicted minimal epitopes.

Article Snippet: 15mer peptides were synthesized by Mimotopes, VIC, Australia.

Techniques: Sequencing

Journal: PLOS ONE

Article Title: Human responses to the DNA prime/chimpanzee adenovirus (ChAd63) boost vaccine identify CSP, AMA1 and TRAP MHC Class I-restricted epitopes

doi: 10.1371/journal.pone.0318098

Figure Lengend Snippet: Cohort CAT: FluoroSpot IFN-γ and GzB responses for protected participant v12 (HLA A*02:02 (A02)/A*32:01 (A01), B*35:01 (B07)/B*44:03 (B44) to AMA1 Ap8 15mer peptide and synthesized predicted epitope.

Article Snippet: 15mer peptides were synthesized by Mimotopes, VIC, Australia.

Techniques: Synthesized, Sequencing

Journal: PLOS ONE

Article Title: Human responses to the DNA prime/chimpanzee adenovirus (ChAd63) boost vaccine identify CSP, AMA1 and TRAP MHC Class I-restricted epitopes

doi: 10.1371/journal.pone.0318098

Figure Lengend Snippet: Cohort CAT: FluoroSpot IFN-γ and GzB responses for protected participant v24 (HLA A*02:01 (A02)/A*30:01 (A01A03), B*35:01 (B07)/B*42:02 (B07) to AMA1 Ap1, Ap3 and Ap9 peptide pools, 15mer peptides, and synthesized predicted epitopes.

Article Snippet: 15mer peptides were synthesized by Mimotopes, VIC, Australia.

Techniques: Synthesized, Sequencing

Journal: PLOS ONE

Article Title: Human responses to the DNA prime/chimpanzee adenovirus (ChAd63) boost vaccine identify CSP, AMA1 and TRAP MHC Class I-restricted epitopes

doi: 10.1371/journal.pone.0318098

Figure Lengend Snippet: Cohort CAT: FluoroSpot IFN-γ and GzB responses for non-protected participant v33 ( HLA A*23:01 (A24)/A*33:03 (A03), B*51:01 (B07)/B*58:01 (B58) to AMA1 Ap4, Ap8 and Ap10 peptide pools, 15mer peptides, and synthesized predicted epitopes.

Article Snippet: 15mer peptides were synthesized by Mimotopes, VIC, Australia.

Techniques: Synthesized, Sequencing

Journal: PLOS ONE

Article Title: Human responses to the DNA prime/chimpanzee adenovirus (ChAd63) boost vaccine identify CSP, AMA1 and TRAP MHC Class I-restricted epitopes

doi: 10.1371/journal.pone.0318098

Figure Lengend Snippet: Cohort CAT: FluoroSpot IFN-γ responses for non-protected participant v35 (HLA A*02:01 (A02)/A*02:06 (A02), B*39:05 (B27)/B*58:01 (B58) to AMA1 Ap8 peptide pool, 15mer component peptides, and synthesized predicted epitopes within active 15mers.

Article Snippet: 15mer peptides were synthesized by Mimotopes, VIC, Australia.

Techniques: Synthesized, Sequencing